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KMID : 0380619930250060689
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Korean Journal of Food Science and Technology
1993 Volume.25 No. 6 p.689 ~ p.693
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Characterization of α - galactosidase from Bifidobacterium sp . Int - 57
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Abstract
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a-galactosidase releases galactoside from raffinose and stachyose which are the major sugars in soybean, Although raffinose and stachyose were known as flatulence factors, these sugars were recently claimed as bifidus factors. In this experiment we studied the properties of α-galactosidase and its production from Bifidobacterium sp. Int-57. Int-57 produced higher level of α-galactosidase than other intestinal bacteria. The production of α-galactosidase was greater when grown on raffinose compared with other carbohydrates tested. Partially purified α-galactosidase was obtained after sonication of harvested cell pellet followed by DEAE-cellulose chromatography and Sepharose CL-6B gel filtration, and assayed using PNP-α-galactoside as a substrate. Optimum pH for activity was 7.0 and optimum temperature was 40℃ . At 5 mM concentration of metal ions, CoCl₂ and CuCl₂ inhibited the enzyme activity by 33% and 21% respectively. The enzyme was shown to hydrolyse genuine substrates, i.e. raffinose and stachyose.
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KEYWORD
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